Various lysates were subjected to SDS PAGE followed by western blot with 14410-1-AP (TAB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human malignant melanoma using 14410-1-AP (TAB2 antibody) at dilution of 1:100 (under 40x lens).
Immunofluorescent analysis of HepG2 cells, using MAP3K7IP2 antibody 14410-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-TAB2 (IP:14410-1-AP, 4ug; Detection:14410-1-AP 1:600) with HepG2 cells lysate 3600ug.
1X10^6 HepG2 cells were stained with 0.2ug TAB2 antibody (14410-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
